Fig. 7. Intracellular Ca²⁺ sensitivity of Maxi-Cl currents and its inhibition by knockdown of S100a10 in C127 cells. A. Time courses of Maxi-Cl channel activation after patch excision from C127 cells recorded at +25 mV under conditions in which the intracellular side of patch membrane was exposed to AISs containing free Ca²⁺ at given concentrations (n=5-7). B. Concentration-dependent activation of Maxi-Cl channels by intracellular free Ca²⁺. The nPo (mean number of channels open) values are plotted as a function of free Ca²⁺ concentration. Each circle point represents the mean ± SEM (vertical bar). C, D, E, F. Representative time courses of Maxi-Cl current activation after patch excision from C127 cells transfected with non-targeting (Mock control) siRNA (C, E) and S100a10-specific siRNA (D, F). During these records, alternating pulses from 0 to ±25 mV were applied in the presence (+Ca: C, D) or absence (-Ca: E, F) of 10 µM free Ca²⁺. G. Summary of the effects of ±10 µM free Ca²⁺ for the mock control (MC) and S100a10-specific siRNA knockdown (KD) on the steady-state nPo value recorded at +25 mV. Each column represents the mean ± SEM (vertical bar). *p<0.05 (ANOVA) vs MC+Ca.